Tryptase and Chymase Expression Differences of Mast Cells in Prostatic Adencarcinomas

Abstract

Objective Mast cells (MCs) have been shown to have various roles in many tumors. MCs play roles in tumor progression via increasing angiogenesisand lymphangiogenesis. According to the presence of tryptase and chymase granules, mast cells are two varieties containing tryptasechymaseand containing tryptase. The aim of this study was to investigate the presence and distribution of these two mast cell types in PCain terms of their tryptase and chymase content.( Sakarya Med J, 2018, 8(2):229-234 )Materials andMethodsIn this study, immunohistochemical analysis of tryptase and chymase expression was performed retrospectively in 134 patients who havediagnosed PCa histopathologically, at the Department of Pathology of Faculty of Medicine, **** University Educational Research Hospital,between 2012 and 2014. Immunohistochemisrty experiment was done on the appropriate paraffi n blocks by using mast cell tryptase andchymase antibodies. The research fi ndings were evaluated statistically and accepted as P <0.05 level.Results It has been observed that Mast Cell Chymase (MCTC) accumulates in intratumoral areas more than peritumoral areas (respectively; mean:14,28 ± 14,06, mean: 12,12 ± 12,56). Mast Cell Tryptase (MCT) accumulates more than MCTC in intratumoral and peritumoral areas(P<0,001). MCT was observed to be more frequent in peritumoral areas than in intratumoral areas (respectively; mean: 33,94 ± 20,09,mean:33,50 ± 18,65). MCT and MCTC compared with regard to intratumoral and peritumoral areas. There wasn’t a signifi cant differencewith MCT (p=0.723). However, MCTC was found the intratumoral area more than the peritumoral area (p=0.007).Conclusion Mast cells numbers were increased around prostatic adenocarcinoma microenvironment. MCTC was tended to locate intratumoralwhereas MCT was tended to the located peritumoral area.