Stimulation of 5-Hydroxytriptamine 7 Receptor by LP-211 Boosts Angiogenic Response

Abstract

Background and Objective: The 5-hydroxytriptamine type 7 receptors (5-HT7Rs) belong to the serotonin receptor family and modulate many processes in organism and are also found in membrane of endothelial cells (ECs). The LP-211 is a selective agonist of 5-HT7Rs. The purpose of this study was to investigate the effects of activated 5-HT7Rs by LP-211 on regulation of angiogenesis. Materials and Methods: The effect of LP-211 was tested in vitro by cell proliferation and matrigel assays and in vivo by chick chorioallantoic membrane (CAM) assay. Results: Cell proliferation assay showed that LP-211 had a positive effect on EC proliferation. The LP-211 activated migration of ECs and promoted angiogenesis in matrigel assay in vitro. Stimulation of 5-HT7R by LP-211 caused a significant increase in angiogenic response compared to the control group in CAM assay in viva. The LP-211, selective agonist of 5-HT7R, was found to have an angiogenic effect in vitro and in viva. Conclusion: The 5-HT7Rs can be considered to have an important role in EC functions and angiogenesis. Thus, 5-HT7Rs may be a potential target for the therapeutic interventions aimed to regulate the process of angiogenesis in diseases such as varicose veins, peripheral vascular diseases or vasculature of solid tumors. The LP-211 may be a candidate to improve the ineffective circulation in related disorders.