Antibacterial effectiveness of combined sodium hypochlorite and diode laser therapy with different parameters against S. Aureus biofilm

Abstract

Aim: In this study, we aimed to assess the antibacterial efficacy of both single and combined sodium hypochlorite (NaOCl) and diode laser on Staphylococcus aureus (S. aureus) biofilm.Material and Methods: Eighty-four premolars were randomly assigned to groups. The Experimental groups were as follows: group 1; 5.25% NaOCl; groups 2-4; diode laser (0,7; 1,5; 2,5 W); Groups 5-7; combined laser and NaOCl. Statistical evaluations were made by Kruskal-Wallis and Mann-Whitney U tests.Results: The 2.5 W diode laser showed a high potential for elimination of S.aureus, similar to NaOCl (p>0.05). 0,7 W and 1,5 W diode lasers showed less antibacterial activity than the other groups (p<0.05). There was no difference between single and combined applications (p>0.05).Discussion: Diode laser application did not show a synergistic effect with NaOCl. The 2,5 W laser parameter was also found to be sufficient on its own. The effectiveness of laser irrigation has been shown to depend on parameters.