Inhibition effects of selected thiophene-2-sulfonamides on lactoperoxidase

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dc.authorid0000-0002-0869-8517
dc.contributor.authorKöksal, Zeynep
dc.date.accessioned2025-05-10T19:44:49Z
dc.date.issued2021
dc.departmentİstanbul Medeniyet Üniversitesi
dc.description.abstractLactoperoxidase (LPO, E.C.1.11.1.7) is a natural antibacterial agent which is secreted from salivary, mammary, and other mucosal glands. It is one of the crucial enzymes in biological systems, so protection of LPO activity is extremely important for the immune system. Within the scope of this study; in vitro effects of some thiophene-2-sulfonamide derivatives (1a-7a) on bovine milk LPO enzymatic activity were investigated. LPO was purified from the Sepharose-4B-L-tyrosine-5-amino-2-methylbenzenesulfonamide column prepared using affinity chromatography technique with a yield of 169.66 EU/mg specific activity in 452.44 times. As a result, 5-(2-thienylthio) thiophene-2-sulfonamide demonstrated the strongest inhibition impact among these compounds. This molecule has shown a competitive inhibition and it was determined that the IC50 value was 3.4 nM and the Ki value was 2 +/- 0.6 nM.
dc.description.sponsorshipIstanbul Medeniyet University, Turkey [F-GAP-2017-1167]
dc.description.sponsorshipThis research is supported by Istanbul Medeniyet University, Turkey, under Grant F-GAP-2017-1167.
dc.identifier.doi10.1080/01480545.2019.1600532
dc.identifier.endpage364
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue4
dc.identifier.pmid31010344
dc.identifier.scopus2-s2.0-85064709810
dc.identifier.scopusqualityQ1
dc.identifier.startpage359
dc.identifier.urihttps://doi.org/10.1080/01480545.2019.1600532
dc.identifier.urihttps://hdl.handle.net/20.500.14730/11060
dc.identifier.volume44
dc.identifier.wosWOS:000470354700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKöksal, Zeynep
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250302
dc.subjectLactoperoxidase
dc.subjectpurification
dc.subjectthiophene-2-sulfonamide
dc.subjectkinetics
dc.subjectenzyme inhibition
dc.titleInhibition effects of selected thiophene-2-sulfonamides on lactoperoxidase
dc.typeArticle

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